Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside CAS:24404-53-3

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside biasane digunakake minangka substrat kanggo enzim β-galactosidase.Nalika β-galactosidase tumindak, senyawa kasebut dihidrolisis, nyebabake produksi p-nitrophenol utawa o-nitrophenol, sing nduweni werna kuning sing gampang dideteksi.Iki ndadekake migunani minangka substrat kanggo tes sing ngukur aktivitas β-galactosidase.

Salah sawijining aplikasi umum Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ana ing tes gen reporter.Ing tes kasebut, gen sing digandhengake karo gen reporter, kayata gen beta-galactosidase.Aktivitas gen reporter banjur dipantau kanthi ngukur aktivitas β-galactosidase nggunakake Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside minangka substrate.Iki ngidini peneliti sinau ekspresi lan regulasi gen.

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside uga bisa digunakake kanggo screening inhibitor utawa aktivator β-galactosidase.Kanthi nguji macem-macem senyawa utawa calon obat, peneliti bisa ngenali molekul sing ngowahi aktivitas enzim iki, sing bisa duwe aplikasi terapeutik ing penyakit utawa kondisi tartamtu.

Kajaba iku, senyawa iki bisa digunakake ing pemurnian protein.β-galactosidase asring digabungake karo protein sing disenengi kanggo nggampangake deteksi lan pemurnian.Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside bisa digunakake minangka substrat kanggo nyaring lan ngresiki protein fusi kasebut kanthi ndeteksi aktivitas β-galactosidase.